947 Changes in the cutaneous microbiome in keratinocyte carcinoma and their association with clinicopathological characteristics

Recently, researchers have reported that the microbiome is involved in development of cancer. Although, keratinocyte carcinoma (KC), comprised squamous cell (SCC) and basal (BCC), most common cancer, little known about association between cutaneous (CM) carcinogenesis KC, especially Asians. The aim this study to investigate KC-associated changes CM by comparing microbiota composition diversity paired tumor adjacent normal tissues. We collected KC skin tissues from surgically resected 30 patients (13 SCC 17 BCC patients). performed 16S rRNA amplicon gene sequencing all 60 samples assess profiles. alpha beta metrics were not significantly different tissue tissue. However, absolute relative compositions different. Both compositional functional analyses indicated microbes possibly associated with development. There a unique distribution epidermis dermis compared superficial skin. propose microbiota, which less affected environmental factors, might present pattern contributing KC.